Which genetic modification technique is commonly used to create transgenic organisms?

Recombinant DNA technology is the widely utilized technique for creating transgenic organisms, which are organisms that contain a gene or genes that have been artificially inserted instead of the organism acquiring them through reproduction. This process involves combining DNA molecules from different sources and inserting them into a host organism to express desired traits.

The core of recombinant DNA technology involves the use of restriction enzymes to cut DNA at specific sequences, allowing for the insertion of foreign DNA into a plasmid or a viral vector. Once the recombinant DNA is formed, it can be introduced into the target organism’s cells through various methods such as transformation, transduction, or electroporation. This facilitates the expression of new traits, such as resistance to pests, enhanced nutritional content, or increased growth rates.

While other techniques such as CRISPR-Cas9 and RNA interference are important in genetic research and modifications, they work differently. CRISPR-Cas9 is primarily a gene-editing tool that allows for precise alterations to the genome, but it doesn’t necessarily involve the insertion of foreign genes to create a transgenic organism in the same way recombinant DNA does. Cloning focuses on producing identical copies of an organism or a cell rather than introducing new genes. Gene editing via RNA interference is a method